Authors: Uwe Bornscheuer, Thomas Oiffer, Friedemann Leipold, Philipp Süss, Daniel Breite, Jan Griebel, Muhammad Khurram, Yannick Branson, Erik de Vries, Agnes Schulze, Christiane A. Helm, and Ren Wei
A Journal of the Gesellschaft Deutscher Chemiker, Angewandte Chemie
Abstract
Polyethylene (PE) is the most commonly used plastic type in the world, contributing significantly to the plastic waste crisis. Microbial degradation of PE in natural environments is unlikely due to its inert saturated carbon-carbon backbones, which are difficult to break down by enzymes, challenging the development of a biocatalytic recycling method for PE waste. Here, we demonstrated the depolymerization of low-molecular-weight (LMW) PE using an enzyme cascade that included a catalase-peroxidase, an alcohol dehydrogenase, a Baeyer Villiger monooxygenase, and a lipase after the polymer was chemically pretreated with m-chloroperoxybenzoic acid (mCPBA) and ultrasonication. In a preparative experiment with gram-scale pretreated polymers, GC-MS and weight loss determinations confirmed ~27% polymer conversion including the formation of medium-size functionalized molecules such as ω-hydroxy acids and α,ω-carboxylic acids. Additional polymer property analyses using AFM showed that enzymatic depolymerization reduced the particle sizes of this mCPBA- and enzyme-treated LMWPE. This multi-enzyme catalytic concept with distinct chemical steps represents a unique starting point for future development of bio-based recycling methods for polyolefin waste.
https://onlinelibrary.wiley.com/doi/10.1002/anie.202415012
Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by Haloferax mediterranei Using Candy Industry Waste as Raw Materials
by
Lorena Simó-Cabrera
Salvador García-Chumillas
Sergio J. Benitez-Benitez
Verónica Cánovas
Fuensanta Monzó
Carmen Pire
Rosa María Martínez-Espinosa
- Biochemistry, Molecular Biology, Edaphology and Agricultural Chemistry Department, Faculty of Science, University of Alicante, Carretera San Vicente del Raspeig s/n-03690 San Vicente del Raspeig, E-03690 Alicante, Spain
- Multidisciplinary Institute for Environmental Studies “Ramón Margalef”, University of Alicante, Ap. 99, E-03080 Alicante, Spain
- Technological Centre of Footwear and Plastic of the Region of Murcia (CETEC) Avda, Europa 4-5, E-30840 Alhama de Murcia, Spain
- Cetec Biotechnology, Avda, Europa 4-5, E-30840 Alhama de Murcia, Spain
This article belongs to the Special Issue Advances in Polyhydroxyalkanoate (PHA) Production, 4th Edition)
PHBV cycle of life using waste as a starting point: from production to recyclability
Salvador García-Chumillas 1, Teresa Guerrero-Murcia 2,
María Nicolás-Liza 1, Fuensanta Monzó 2, Alexandra Simica 3,4,
Lorena Simó-Cabrera 3,4 and Rosa María Martínez-Espinosa 3,4*
1 Cetec Biotechnology S. L., Avda, Alhama deMurcia, Spain, 2 Technological Centre of Footwear and Plastic of the Region of Murcia (CETEC) Avda, Alhama de Murcia, Spain, 3 Biochemistry and Molecular Biology and Edaphology and Agricultural Chemistry Department, Faculty of Sciences, University of Alicante, Alicante, Spain, 4 Multidisciplinary Institute for Environmental Studies “Ramón Margalef”,
University of Alicante, Alicante, Spain
upPE-T project: PHBV leads the development of biodegradable plastics
The Innovation Platform - Issue 16
A deep review of the start of the art regarding bioplastic production by Haloarchaea
Authors:
Simó-Cabrera, L. Carmen Pire and Rosa María Martínez-Espisonsa from Universidad de Alicante .
Salvador García-Chumillas y Verónica Cánovas from CETECBIO,
Alejandro Arribas y Fuensanta Monzó from CETEC.
(2021) Haloarchaea as Cell Factories to Produce Bioplastics. Marine Drugs, 19(3), 159.
https://www.researchgate.net/publication/350154066_Haloarchaea_as_Cell_Factories_to_Produce_BioplasticsBiosensor and chemo-enzymatic one-pot cascade applications to detect and transform PET-derived terephthalic acid in living cells
Wei, R., Pfaff, L. and Bornscheuer, U.T. from the University of Greifswald
Iscience, 25(5), 104326
https://www.sciencedirect.com/science/article/pii/S2589004222005971Molecular and biochemical differences of the tandem and cold-adapted PET hydrolases Ple628 and Ple629, isolated from a marine microbial consortium
Wei, R., Pfaff, L. and Bornscheuer, U.T. from the University of Greifswald
Frontiers in Bioengineering and Biotechnology, 2022, p. 1010.
https://doi.org/10.3389/fbioe.2022.930140Multiple substrate binding mode‐guided engineering of a thermophilic PET hydrolase
Wei, R., Pfaff, L. and Bornscheuer, U.T. from the University of Greifswald
ACS Catal. 2022, 12, 15, 9790–9800
https://doi.org/10.1021/acscatal.2c02275
